The Test centre for railway rolling stock and containers VÚKV a. s., Prague, carried out the tests of electric locomotive Škoda 109E on the reference tracks of ÖBB Infastruktur in Austria. Lateral and vertical forces (Y and Q) on two wheelsets of one bogie, accelerations in the locomotive body and on the bogie frame, speed and cant deficiency were measured. The tests were carried out between the Linz – St. Valentin, Innsbruck – Langen am Arlberg, St. Veit an der Glan – Neumarkt in der Steiermark, Unzmarkt – Knittelfeld – St. Michael and Semmering – Gloggnitz stations. The wheel force measuring was carried out by means of measuring wheel sets System VÚKV 3 developed by VÚKV a. s. The system worked reliably without any serious failure. The mentioned tests are necessary for service approval of the locomotive in Austria.